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What is 1x PBS?

Updated: 4/28/2022
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13y ago

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It is just Phosphate buffered saline.

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13y ago
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Mahamat Bosha

Lvl 1
2y ago
PBS: Phosphate-Buffered Saline : is an isotonic solution that is used in many biological research applications. PBS Solution Concentrations: NaCl: 137 mM KCl: 2.7 mM Na2HPO4: 10 mM KH2PO4: 1.8 mM

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Related questions

What does 1x PBS mean?

1X PBS might mean 1 in 10 solution 1/10


When to use 1x or 10x pbs?

Use 1x PBS (phosphate-buffered saline) for routine washing and dilution steps in experiments. Use 10x PBS for preparing a concentrated stock solution that can be easily diluted to make 1x PBS when needed.


What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?

1x PBS buffer typically has a molarity of around 0.01 M. To prepare a 20 mM PBS buffer, you would need to dilute the 1x PBS stock solution with water. For example, to make 1 liter of 20 mM PBS buffer, you would need to mix 2 ml of 1 M PBS stock solution with 98 ml of water.


10x PBS buffer?

10x PBS buffer is a concentrated solution commonly used in biological and biochemical research. It typically contains a mixture of phosphate ions, sodium chloride, and potassium chloride at higher concentrations compared to the standard 1x PBS buffer. When diluted with water to 1x concentration, it can be used for a variety of applications such as cell culture, immunostaining, and protein analysis.


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