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Two's complement is the normal implementation of signed integers, so just take the negative.

int a = 123;

int b = -a; /* b now equals -123 */

The actual implementation of two's complement notation is that, to make something negative, simply invert the bits and then add 1.

12310 = 00000000011110112 (using 16 bit notation)

Invert and you get 111111111000100. Add one and you get 111111111000101, so...

-12310 = 1111111110001012

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