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The only way a biochemical oxygen demand value could be negative is if oxygen increased in your incubation. This could result from several errors, but most likely light was allowed to penetrate your bottle allowing photosynthesis to occur. If everything was done correctly (e.g. insturments are calibrated and working, no errors were made in calculations) you should have at least a 1mg/L difference between initial and final dissolved oxygen readings yielding a positive value.

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Q: Does a biological oxygen demand value would be a negative value?
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Can dissolved oxygen value be a negative value?

No, dissolved oxygen values cannot be negative. Dissolved oxygen levels in water are typically measured in milligrams per liter (mg/L) or parts per million (ppm) and will always be a positive value. Negative values would not make physical sense in this context.


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