To make a 1 to 100 dilution, start by taking 1 part of the concentrated solution and add it to 99 parts of a diluent (usually water or a buffer). For example, if you use 1 mL of the concentrated solution, mix it with 99 mL of the diluent. Ensure thorough mixing to achieve a uniform dilution. This results in a final solution that is 1% of the original concentration.
take 1 ml, add 9 ml water.
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
To make a 1 to 1 dilution, you combine equal volumes of the concentrated solution and a diluent, typically a solvent like water. For example, if you have 10 mL of the concentrated solution, you would add 10 mL of the diluent. Mix thoroughly to ensure a uniform solution. This results in a final volume of 20 mL at half the concentration of the original solution.
To make an 8-fold dilution, you would mix 1 part of the substance you want to dilute with 7 parts of the diluent (usually water or buffer solution). This results in a total of 8 parts, with 1 part being the original substance and 7 parts being the diluent. This dilution reduces the concentration of the original substance by a factor of 8.
take 1 ml, add 9 ml water.
A 100 times dilution of a stock solution means that you are adding 1 part of the stock solution to 99 parts of a diluent (such as water) to make a total volume of 100 parts. This results in a final solution that is 1/100th the concentration of the original stock solution.
You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
The recommended bleach dilution ratio for disinfecting surfaces effectively is 1:100, which means mixing 1 part bleach with 100 parts water.
To make a 1 to 1 dilution, you combine equal volumes of the concentrated solution and a diluent, typically a solvent like water. For example, if you have 10 mL of the concentrated solution, you would add 10 mL of the diluent. Mix thoroughly to ensure a uniform solution. This results in a final volume of 20 mL at half the concentration of the original solution.
It is a mixture of 1 part of the active ingredient (by volume) to 10 parts of the solvent.
100*(1/10) = 10%100*(1/10) = 10%100*(1/10) = 10%100*(1/10) = 10%
Your best bet is a serial dilution, i.e. get 2 10ml volumetric flasks and take 1ml of your solution to be diluted, and make to 10ml with solvent, mix this, then take 1ml of the resulting 10mls and dilute that to 10ml. 2x 10 times dilutions is 100x dilution. Alternatively if you have less to start with, use micro pipettes
You add 9.09ml of stock solution to a volumetric and make it up to 1 litre to get a 110 dilution