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take 1 ml, add 9 ml water.
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
1/10 = 10/100. 10/100 + 1/100 = 11/100
ten 1/10 x 100 = 100/10 = 10/1 = 10
An 8-fold dilution means you are making your solution 1/8 less concentrated. So, if you want to make 100 ml of a solution diluted 8 fold you would do as follows: 1/8=.125 100*.125= 12.5 Mix 12.5 mL of your concentrated solution with 87.5 mL of your solvent (water etc..) to bring the final volume to 100 mL. If you wanted to perform a ten fold dilution you would simply mix 10 mL of your concentrated solution with 90 mL of your solvent. 1/10=.1 100*.1=10
take 1 ml, add 9 ml water.
A 100 times dilution of a stock solution means that you are adding 1 part of the stock solution to 99 parts of a diluent (such as water) to make a total volume of 100 parts. This results in a final solution that is 1/100th the concentration of the original stock solution.
You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
1 dollar = 100 cents = 10*10 cents. So ten lots of 10 cents make 1 dollar.1 dollar = 100 cents = 10*10 cents. So ten lots of 10 cents make 1 dollar.1 dollar = 100 cents = 10*10 cents. So ten lots of 10 cents make 1 dollar.1 dollar = 100 cents = 10*10 cents. So ten lots of 10 cents make 1 dollar.
It is a mixture of 1 part of the active ingredient (by volume) to 10 parts of the solvent.
To prepare a 1:100 dilution in a small volume, mix 1 part solute with 99 parts solvent (such as 1 ml solute with 99 ml solvent). For example, to dilute 1 ml of a substance in 99 ml of water, mix thoroughly to ensure a homogeneous solution. Note that maintaining accurate measurements and thorough mixing are crucial for successful dilution.
You add 9.09ml of stock solution to a volumetric and make it up to 1 litre to get a 110 dilution
100*(1/10) = 10%100*(1/10) = 10%100*(1/10) = 10%100*(1/10) = 10%
1 in 25. (10/250)