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70% alcohol has been found to be more effective in killing microbes.Higher and lower concentration may not be as effective.90% alcohol coagulate proteins in contact.suppose 90% alcohol is poured over one celled organism,the alcohol penetrate the cellwall of the organism.the coagulated protein would then prevent the alcohol further from the cell and no more coagulation then take place.now,if 70% alcohol is added into one celled organism.The diluted alcohol also coagulates the protein, but at a slower rate,so that it penetrates all the cell before coagulation.Then all the cell coagulated and the organism died.
1) take 10gm of gentian violet. then add to them: 2) Alcohol 70% ( H2O).... to 1000 ml
prepare it
90% of 90 = 90*90/100 = 81
90 - 90 = 0