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I have to prepare a 1 to 10 dilution and have a 2ml culture suspension and want to prepare a 1 to 10 dilution of the entire culture.?

Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10


To prepare 300 ml of a 115 dilution from a 13 dilution you need to add ml of the 13 dilution to ml of diluent?

33,4ml


Definition of dilution and dilution factor?

In chemistry and biology, the dilution factor is the total number of unit volumes in which the material is dissolved. As I understand it, the dilution refers to the dilution ratio. If you add 1 part of something to 4 parts of something else, the dilution ratio is 1 to 4. The dilution factor counts all the parts and expresses the same thing as 1 out of 5.


How do you make a 1 to 5 dilution?

To make a 1 to 5 dilution you mix 1 part of your substance with 4 parts water. ie: Mix 1 tablespoon of creamer with 4 tables spoons of coffee, and the coffee is 1/5 creamer now.


How does one prepare a 1100 dilution?

1 part of solution A plus 99 parts solution B


How would you prepare 20 ml of a 1 to 400 dilution?

To prepare 20 ml of a 1:400 dilution, you would mix 20 ml of the original solution with 7980 ml (20 ml x 400) of the diluent (such as water or buffer). This will give you a final solution with a concentration that is 1/400th of the original solution.


If dilution is 4 oz of powder makes 128 gallons how much powder for 1 gallon?

0.03125 ounces.


A 4 percent solution of red blood cells is needed for diagnostic testing in the laboratory what dilution of packed red blood cells does this represent?

1:4


How do you prepare 1mM edta from 0.1M edta-Na2 solution?

You dilute it 1:10, then you take 1 part of that solution and mix it with 9 parts of the diluent. That will make the 1:100 dilution you need, incl. prevention of pipette inaccuracy.


What is the difference between 1 colon 2 and 1 slash 2 dilution?

A 1:2 dilution means mixing 1 part of a substance with 2 parts of solvent or diluent, resulting in a total of 3 parts. A 1/2 dilution involves dividing the original concentration in half, resulting in a 1:1 dilution ratio of the original substance.


How do you do parallel dilution?

Parallel dilution is the dilution of a solution with equal quantity of the same solvent with which the solution is made. e.g., 1mL of 100µg/ml strength aqueous solution can be diluted to 2mL of 50µg/mL strength solution by adding 1mL Water.


How do you prepare 1 is to 100 dilution in small volume?

Your best bet is a serial dilution, i.e. get 2 10ml volumetric flasks and take 1ml of your solution to be diluted, and make to 10ml with solvent, mix this, then take 1ml of the resulting 10mls and dilute that to 10ml. 2x 10 times dilutions is 100x dilution. Alternatively if you have less to start with, use micro pipettes