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What is o-phthaldehyde?
O-phthaldehyde (OPA) is a small fluorscent molecule that reacts with primary amines, predominantly lysines and N-termins amines, in aqueas solutions at room temperature. It is used to determine the total concentration of protein in a sample. OPA can be used within a concentration of 1µg/ml - 1 mg/ml. Because it will react with N-terminus amines, it is a good choice for assays of small peptides that may not have enough amino acids to be detected by Bradford, BCA or Lowry protein assays.
What is the difference between a qualitative and a quantitative assay?
In assays, or medical tests, both qualitative and quantitative tests occur. The qualitative test identifies the presence or absence of the pathogen or toxin (or other molecule) being tested for, while the quantitative test hopes to measure how much of that particular substance is in the body.
How many sums equal 76?
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How much chords can there be in a circle?
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