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Is PCR assays a qualitative or quantitative test?
PCR assays can be both qualitative and quantitative, depending on the method used. Qualitative PCR, often referred to as conventional PCR, detects the presence or absence of a specific DNA sequence. In contrast, quantitative PCR (qPCR or real-time PCR) measures the amount of DNA, providing information on the quantity of the target sequence in a sample. Thus, PCR can serve both purposes based on the specific assay design.
What is o-phthaldehyde?
O-phthaldehyde (OPA) is a small fluorscent molecule that reacts with primary amines, predominantly lysines and N-termins amines, in aqueas solutions at room temperature. It is used to determine the total concentration of protein in a sample. OPA can be used within a concentration of 1µg/ml - 1 mg/ml. Because it will react with N-terminus amines, it is a good choice for assays of small peptides that may not have enough amino acids to be detected by Bradford, BCA or Lowry protein assays.
What is a continuous assay?
A continuous assay is a type of analytical technique that allows for the ongoing measurement of a specific biological or chemical process over time, rather than at discrete intervals. This method enables real-time monitoring of reactions, providing insights into kinetics and dynamic changes. Continuous assays are often used in fields such as biochemistry, pharmacology, and environmental monitoring to enhance the understanding of reaction mechanisms and improve data accuracy. Examples include continuous flow assays and real-time PCR.
What is the difference between a qualitative and a quantitative assay?
In assays, or medical tests, both qualitative and quantitative tests occur. The qualitative test identifies the presence or absence of the pathogen or toxin (or other molecule) being tested for, while the quantitative test hopes to measure how much of that particular substance is in the body.
What is 1 microliter equivalent to?
One microliter (µL) is equivalent to one millionth of a liter, or 0.001 milliliters (mL). It is also equal to 1,000 nanoliters (nL) and can be visualized as a small droplet of liquid. In practical terms, it's commonly used in laboratory settings for measuring small volumes of liquids in experiments and assays.
What is molecular assays?
Molecular assays are laboratory techniques that detect and analyze the genetic material (DNA or RNA) of organisms. These assays are used to identify specific genes, mutations, or pathogens, and are widely used in research, diagnostics, and pharmaceutical development. Examples of molecular assays include polymerase chain reaction (PCR), next-generation sequencing (NGS), and hybridization assays.
What are four different ways scientists get information about the history of species?
1. Comparative genomics assays. 2. Comparative morphology assays. 3. Combining comparative genomics and morphology assays. 4. ...?
In what field of work involves the use of assays?
Assays is a procedure to analyze or quantify a substance in a sample to determine the presence and amount, as well as the potency. The fields that involve the use of assays are environmental biology, laboratory medicine, pharmacology, and molecular biology.
What has the author Mario Serio written?
Mario Serio has written: 'Luminescent Assays'
What was luminol originally used for?
used by biologists in cellular assays for the detection of copper, iron, and cyanide.
What are the advantage of radioimmunoassay?
higher sensitivity, easy signal detection, and well-established, rapid assays
How do we measure ATP?
ATP (adenosine triphosphate) can be measured using various methods, with the most common being bioluminescence assays. These assays involve the enzyme luciferase, which catalyzes a reaction that produces light in the presence of ATP. The intensity of the emitted light is proportional to the amount of ATP present and can be quantified using a luminometer. Other methods include high-performance liquid chromatography (HPLC) and spectrophotometric assays, though bioluminescence is widely preferred for its sensitivity and simplicity.
What are the benefits of using the TaqMan Gene Expression Assays?
TaqMan Gene Expression Assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a FAM or VIC dye label on the 5' end, and minor groove binder (MGB) nonfluorescent quencher (NFQ) on the 3' end.
What are some of the advantages of latex agglutination assays?
Latex agglutination assays offer several advantages, including rapid results, ease of use, and minimal sample preparation. They are highly sensitive and specific, allowing for the detection of various analytes, including antibodies and antigens. Additionally, these assays can be performed on a variety of sample types, making them versatile for clinical and laboratory settings. The visual interpretation of results, often through a color change, simplifies the process for users without extensive training.
What are the type of biochemical assays in HTS?
Check out Inglese et al., Nature (3) 8. August 2007.
What has the author Janette Mary Sullivan written?
Janette Mary Sullivan has written: 'Peptide derivatives for proteinase assays'
What is the significance of NADH absorbance in biochemical assays?
NADH absorbance is significant in biochemical assays because it can be used to measure the activity of enzymes involved in cellular respiration. Changes in NADH absorbance indicate the conversion of NADH to NAD by enzymes, providing valuable information about metabolic processes and enzyme function.
