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This solution contains the detergent sodium dodecyl sulfate (SDS)
which dissolves the cell membrane and denatures proteins. The solution is very alkaline (pH > 12) due to the presence of sodium hydroxide. The high pH aids in denaturing proteins and causes the cleavage
of the phosphate bonds in RNA. This eliminates interference from high
molecular weight RNA during the plasmid purification. Under highly
alkaline conditions, the two strands in non-supercoiled DNA (linear
fragments of chromosomal DNA, relaxed and nicked circular DNA)
separate and are partially removed from solution. However, this does
not occur with supercoiled forms of plasmid DNA because the two
strands are intertwined and entangled in a way that prevents them
from coming apart. Therefore, supercoiled plasmid remains free in
solution.
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Solution of deriving vertex from the quadratic function?
2 AND 9
Does a quadratic function have to have 2 x-values?
If you mean have 2 different real x-value solutions then no.Otherwise a quadratic function will always have 2 solutions, just that they may both be the same value (repeated root) making it seem like there is only one solution value, or non-real (complex) making it seem like it has no solution value.
Is y2 equals x2 a function?
No, because there is more than one solution: y2 = x2 y = ±(x2)1/2 y = ±x Because there are multiple solutions for a single value of x, this does not qualify as a function.
Is 2 a solution to the inequality x 3?
Yes, It is a solution (a+)
Is 1 -2 a function?
1-2 is not a function because there is no defined function, such as f(n)=0n+1-2. Also, it does not contain a variable.
