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How would you make a 1 in 2 dilution?

Updated: 4/28/2022
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10y ago

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Let's use for example only water and vinegar. I want 1 part vinegar to 2 parts water. 1:2 So I would use the same measure, for the example, 1 cup measure. So 1 cup vinegar and 2 cups water. And you have a great cleaning solution.

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Q: How would you make a 1 in 2 dilution?
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In this procedure a 10x buffer is used if you have a 5x buffer and you want a total volume of 200ul what dilution would you perform to achieve the desired 1x concentration of buffer?

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