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Numerical aperture 1.25 medium poser 0.25 filter 520 nm. What is the resolution. Can you distinguish two points that are 330 nm apart as being separate or will they blur into one?
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∙ 15y ago
Yes
Wiki User
∙ 15y ago
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Resolution is the ability to see two objects separate true or false?
true
Will you be able to distinguish two points that are 330 nm apart as being separate or will they blur into one?
If the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.205 respectively and you are supplied with a filter that selects a wavelength of 521 nm then the answer is YES! 520/(1.25 + 0.25) = 347 nm and your two points in question are shorter in distance as they are only 330 nm apart.
Will you be able to distinguish two points that are 250 nm apart as being separate or will they blur into one?
According to Wikipedia (see link), a human eye with excellent acuity can distinguish between lines that are 0.35 mm apart, held at a distance of 1 meter. That is 350 micrometres or 350,000 nm - somewhat larger than the 250 nm in the question!
What is the future tense of separate?
Will separate.
How do you separate steel pins and wooden toothpicks?
Use magnet to separate pins :)
What do you mean by resolving power of telescope?
The "resolving power" of a telescope is a measure of the ability of a telescope to distinguish between two separate objects that appear to be very close together in the sky.
What term describes the ability of a microscope to distinguish two objects as being separate?
resolution
Four assessments of the quality of the image being seen using a light microscope?
[1] Brightness - How light or dark is the image? Brightness is related to the illumination system and can be changed by changing the voltage to the lamp (rheostat) and adjusting the condenser and diaphragm/pinhole apertures. Brightness is also related to the numerical aperture of the objective lens (the larger the numerical aperture, the brighter the image).[2] Focus - Is the image blurry or well-defined? Focus is related to focal length and can be controlled with the focus knobs. The thickness of the cover glass on the specimen slide can also affect your ability to focus the image -- it can be too thick for the objective lens. The correct cover-glass thickness is written on the side of the objective lens.[3] Resolution - How close can two points in the image be before they are no longer seen as two separate points? Resolution is related to the numerical aperture of the objective lens (the higher the numerical aperture, the better the resolution) and the wavelength of light passing through the lens (the shorter the wavelength, the better the resolution).[4] Contrast - What is the difference in lighting between adjacent areas of the specimen? Contrast is related to the illumination system and can be adjusted by changing the intensity of the light and the diaphragm/pinhole aperture. Also, chemical stains applied to the specimen can enhance contrast.
What does the term resolving power refer to?
The question is about the resolving power of optical instruments like telescope and microscope.It is the ability of the instrument to resolve the images of two points that are close to each other. If dθ is the angular separation, resolving power is given by the formulaR = 1/dθ = D/1.22 λ where Dis the aperture of the objective; λ is the wavelength of the light .
List the four assessments of the quality of the image being seen using a light microscope?
[1] Brightness - How light or dark is the image? Brightness is related to the illumination system and can be changed by changing the voltage to the lamp (rheostat) and adjusting the condenser and diaphragm/pinhole apertures. Brightness is also related to the numerical aperture of the objective lens (the larger the numerical aperture, the brighter the image).[2] Focus - Is the image blurry or well-defined? Focus is related to focal length and can be controlled with the focus knobs. The thickness of the cover glass on the specimen slide can also affect your ability to focus the image -- it can be too thick for the objective lens. The correct cover-glass thickness is written on the side of the objective lens.[3] Resolution - How close can two points in the image be before they are no longer seen as two separate points? Resolution is related to the numerical aperture of the objective lens (the higher the numerical aperture, the better the resolution) and the wavelength of light passing through the lens (the shorter the wavelength, the better the resolution).[4] Contrast - What is the difference in lighting between adjacent areas of the specimen? Contrast is related to the illumination system and can be adjusted by changing the intensity of the light and the diaphragm/pinhole aperture. Also, chemical stains applied to the specimen can enhance contrast.FROM VLA hacker
What is meant by limit of resolution?
It is defined as the capacity of an optical system to resolve point objects as separate objects. Larger the aperture of optical system greater will be its limits of resolution.
What are synonyms of differentiate?
distinguish, separate, discriminate, contrast, tell apart
What does the root word cern mean?
Distinguish, decide, sift, separate
What was the importance of Lahore Resolution?
In Lahore resolution first time Muslims of subcontinent clearly demand for a separate country for the Muslims..this resolution gave a new spirit among Muslims as separate nation...through this resolution Muslims united under the leadership of quad's and achieve their goal...this resolution play a roll of back bone in Pakistan movement...
What is the function of a nuclear envelope that is easy to understand?
It is to separate and distinguish the nucleoplasm from the cytoplasm.
Is the resolution the ability to see two objects as separate?
Yes. The closer two objects you can still see as separate, the greate the resolution. Resolution may be measured in units of length, such as micron, or (for telescopes that watch outer space) in angular units, such as arc-seconds.
Magnification without increased resolution is not useful for studying specimens. why?
Mere magnification without added detail is scientifically useless, just as endlessly enlarging a small photograph may not reveal any more detail, but only larger blurs. The usefulness of any microscope is that it produces better resolution than the eye. Resolution is the ability to distinguish two objects as separate entities, rather than seeing them blurred together as a single smudge.
