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Well, sort of. The Chi-square distribution is the sampling distribution of the variance. It is derived based on a random sample. A perfect random sample is where any value in the sample has any relationship to any other value. I would say that if the Chi-square distribution is used, then every effort should be made to make the sample as random as possible. I would also say that if the Chi-square distribution is used and the sample is clearly not a random sample, then improper conclusions may be reached.

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Q: Does use of chi-square demand a random sample?
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What is the difference between random and stratified sample in the survey method?

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