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take 1 ml, add 9 ml water.
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
what is dilution rate for glycos
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
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