You can give hundreds of examples, but a single counterexample shows that natural numbers are NOT closed under subtraction or division. For example, 1 - 2 is NOT a natural number, and 1 / 2 is NOT a natural number.
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They are closed under all except that division by zero is not defined.
To be closed under an operation, when that operation is applied to two member of a set then the result must also be a member of the set. Thus the sets ℂ (Complex numbers), ℝ (Real Numbers), ℚ (Rational Numbers) and ℤ (integers) are closed under subtraction. ℤ+ (the positive integers), ℤ- (the negative integers) and ℕ (the natural numbers) are not closed under subtraction as subtraction can lead to a result which is not a member of the set.
Integers are the natural numbers (counting numbers: 1,2,3,etc.), and their negative counterparts, and zero. The set of Integers is closed for addition, subtraction, and multiplication, but not division. Closed means that the answer will be a part of the set. Example: 1/3 (1 divided by 3 equals one third) is not an integer, even though both 1 and 3 are integers.
Subtraction.
It cannot be closed under the four basic operations (addition, subtraction, multiplication, division) because it is indeed possible to come up with two negative irrational numbers such that their sum/difference/product/quotient is a rational number, indicating that the set is not closed. You will have to think of a different operation.