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Q: How do you prepare 50 ml of 1M tris?
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How do you prepare 0.5M Tris-HCL pH 7.2?

for 1L 121.1 gr tris base 80 ml HcL 920 ml Distilled Water


How do you make 1X TE buffer?

10 mM Tris pH 7.5 and 1mM EDTA pH 8.0 For 1 L : 10 mL of 1M Tris-Cl pH 7.5 and 2 mL of 500mM EDTA pH 8.0


How do you make 62.5 mM Tris-HCl?

mix 6,25 ml of 1 M tris in a total of 100 ml


How do you prepare Salkowski reagent?

Mix 2 ml 0.5M FeCl3 and 48 ml water and 50 ml 70% perchloric acid.


How many Ml of 14.5 M NH3 are needed to prepare 2.00 L of a 1.00 M solution?

M V = M' V' 1M x 2L = 14.5 M x V' V' = 138 mL


How do you prepare 500cm3 of NaHCO3 solution of 1M concentration?

Weighting half value of 23+1+12+(3x16) = 84/2 = 42 gram NaHCO3 gives you 0.5 mole of it, to be dissolved in about 200 mL and there after to be filled up to 500 mL (= 500 cm3) will result in a 0.5 mol / 500 mL = 0.5 mol / 0.5 L = 1 mol/L = 1M NaHCO3


How do you prepare 50 percent solution of potassium carbonate?

Dissolve 50 g of potassium carbonate in 100 mL of water at 20 0C.


Is 1000 ml greater than 1000 cm?

They cannot be interconverted. A milliliter is a unit of volume while a centimeter is a unit of distance. a milliliter is equal to a cubic centimeter, however.


How do you prepare 1 molar tris?

To make 1.00 litre of a 1.00 M Tris buffer you take 121.14 g Tris and dissolve it by adding water up to 1.00 L (the molar mass of Tris is 121.14 g/mol, that's why) There is a point where you need to set the pH so, its wise to dissolve the given amount in 700 ml ddH2O, setting the pH to 7.5-8.0 using Conc. HCl and then making up the final volume to a litre. filter with 0.5 micron filter and autoclave.


What is the concentration of H3O plus in a solution that has 5 mL of water and 5 mL of 1M HCl?

.5M


How to prepare glycine stock solution of 0.2 m?

The molecular weight of glycine is 75.06 So for 50 ml of 0.2M glycine solution, Weigh 0.75 gram of glycine, and add 50 ml of DI water.


How do you prepare a 50 percent of NaOH solution?

Dissolve slowly 50 g NaOH in 100 mL water; advertisement: sodium hydroxide solution is dangerous !