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How much pure acid should be added to 50 ml of a 10 percent acid solution to give a 50 percent acid solution?
A 50 ml solution that is 10% acid will consist of 5 ml of acid (10% of the volume) and 45 ml of water (90% of the volume). You're not adding any water, but you want to add enough acid to make a solution that is 50% acid and 50% water. You will need to have a total of 45 ml of acid in the mixture to make it a 50/50 solution, since the amount of water is also 45 ml. You have 5 ml in there already, so you would need to add 40 ml of acid. That would make a total 90 ml solution that is 50% water (45 ml) and 50% acid (45 ml).
How much solvent is needed to make 200 ml of 50 percent rubbing alcohol?
200 ml X .50 = 100 ml
How many 355 ml does it take to fill a 1.0-m cubed tank?
1m = 100cm 1m3 = 1m x 1m x 1m = 100cm x 100cm x 100cm = 1000000cm3 1ml = 1cm3 => 355ml = 355cm3 In 1m3 to find the number of 355ml, divide by 355ml: 1m3 / 355ml = 1000000cm3 / 355cm3 ~= 2816.9
How many drops per minute in 50 ml and 10 drops per ml?
50*10 = 500 drops.
I have to prepare a 1 to 10 dilution and have a 2ml culture suspension and want to prepare a 1 to 10 dilution of the entire culture.?
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
How do you prepare 20 mM Tris base of 50ml solution?
To prepare a 20 mM Tris base solution of 50 mL, you would need to calculate the amount of Tris base needed using its molar mass and molarity formula (Molarity = moles of solute / liters of solution). Once calculated, weigh out the required amount of Tris base and dissolve it in deionized water to make a final volume of 50 mL. Mix thoroughly to ensure uniform distribution.
How do you prepare 0.1M tris HCl?
To prepare 0.1M Tris-HCl, dissolve 1.21 g of Tris (molecular weight 121.14 g/mol) in distilled water to make 100 mL of solution. Adjust the pH to 7.4 using HCl.
How do you make 1X TE buffer?
10 mM Tris pH 7.5 and 1mM EDTA pH 8.0 For 1 L : 10 mL of 1M Tris-Cl pH 7.5 and 2 mL of 500mM EDTA pH 8.0
How do you prepare 0.5M Tris-HCL pH 7.2?
To prepare 0.5M Tris-HCl pH 7.2, first calculate the amount of Tris base needed using the molecular weight of Tris (121.14 g/mol). Then dissolve the calculated amount of Tris base in water, adjust the pH to 7.2 using HCl while monitoring with a pH meter, and make up the final volume.
How do you prepare 10 ml of 1M naoh?
To prepare 10 mL of 1M NaOH solution, you would need to dissolve 0.4 grams of NaOH pellets in 10 mL of distilled water. Make sure to wear appropriate personal protective equipment such as gloves and goggles when handling NaOH as it is a corrosive substance.
How can you prepare 1M H2SO4?
To prepare 1M H2SO4 solution, you would need to dilute concentrated sulfuric acid (approximately 18M) by adding the appropriate amount of water. To make 1L of 1M H2SO4 solution, you would mix approximately 55.5 mL of concentrated sulfuric acid with about 944.5 mL of water in a volumetric flask while taking proper safety precautions.
How would you prepare 500 mL of a 1M solution of acetic acid -HC2H3O2- from a stock solution of 6M of acetic acid?
You would need to dilute the 6M acetic acid solution by adding the appropriate volume of water. To prepare 500 mL of 1M solution, you would need to take (1/6)th of the volume of the 6M solution, which is (1/6) x 500 mL = 83.33 mL of the 6M solution. Dilute this with water to reach a final volume of 500 mL.
How do you make 62.5 mM Tris-HCl?
To make 62.5 mM Tris-HCl solution, you would need to mix the appropriate amount of Tris base and HCl to achieve a final concentration of 62.5 mM. The calculation involves considering the molecular weights of Tris and HCl to determine the amount needed to make the desired concentration.
How would you prepare 100 ml of a 5 percent dextrose solution from a 50 percent dextrose solution?
To prepare 100 ml of a 5% dextrose solution from a 50% dextrose solution, you would use the formula: C1V1 = C2V2. You will need 10 ml of the 50% solution (C1) and dilute it with 90 ml of water (V1) to obtain the desired 100 ml of 5% dextrose solution.
How do you prepare 50 percent solution of potassium carbonate?
To prepare a 50% potassium carbonate solution, you would mix equal parts of potassium carbonate powder with water. For example, to make 100mL of 50% solution, you would mix 50g of potassium carbonate with 50mL of water. Stir until the powder is fully dissolved to achieve the desired concentration.
How do you prepare 0.1M tris HCl buffer of pH 8?
Tris(hydroxymethyl)aminomethane (Tris) has a molecular weight of 121.14 g/mol. 50 mM = 0.050 mol/L (x 121.14 g/mol) = 6.057 g/L To prepare a 1L solution first weigh out 6.057 g Tris Add roughly 70% of final volume of water (i.e. 700 mL) Use a pH-meter to measure the pH of the solution Lower the pH of the solution to 7.2 using undiluted HCl Use a measuring cylinder or volumetric flask to make the volume up to 1000 mL If you add too much HCl you need to add more Tris and then recalculate the amount of water that you need add. In this case, every 1 g of Tris requires 165 mL of water to be added.
How do you prepare 1 molar tris?
To prepare 1 M Tris buffer (Tris(hydroxymethyl)aminomethane), dissolve 121.1 g of Tris base in deionized water and adjust the pH to 7.4 with concentrated HCl. Make up the final volume to 1 liter with water. Mix well and then adjust the final pH if needed.
